Add two teaspoons of water to liquefy the strawberry pulp. Strawberry dna extraction institute for molecular bioscience. This lab comes to the rescue with a fun and interactive way of extracting dna from the humble strawberry. Dna can be extracted from cells by a simple technique with household chemicals, enabling students to see. Jun 22, 2018 dna extracting solution mix about 1 cup of dish detergent and 14 cup salt into 4. For dna extraction, detergent is used to lyse the cell so that dna is released into the solution. If you wanted to extract dna from a living person, which cells would you use and why. Our company with artistic authors possess excellent capabilities inside spoken along with created transmission, which in turn convert to the information you may not uncover at any place else. Strawberry dna extraction lab research paper 667 words. Although concentrations were low, clear bands were still obtained from the amplifications. Strawberry dna extraction lab utah state university pdf.
I split my students into table groups of 3 and 4 for this lab. At the end of class, i ask the students whether they preferred the online extraction or the hands on lab. Deoxyribonucleic acid dna is a long molecule that contains the genetic instructions used in the development and functioning of all known living organisms, including the plants we eat. Strawberry dna extraction lab dna carries the genetic code for all living organisms, including humans and strawberries. The dna was extracted due to the dishwashing liquiddetergent busts open the cells of the fruit, releasing the dna and the salt helps as it ensures that the proteins in the cells are not separated from the rest of the. Welcome to the fifteenth episode of season 2 of the sci guys. Add 10 ml of the dna extraction buffer and mash the strawberry and buffer for about one minute.
Resea the bag and gently smash for another minute avoid making too many soap bubbles. Please list two reasons why a scientist might want to study the dna of. Oct 21, 2014 the purpose of this lab was to extract dna from a strawberry. Dna is a double stranded macromolecule composed of nucleotide bases pairing adenine with thymine and guanine with cytosine. Of which becoming stated, we all supply you with a a number of very simple still beneficial articles and web templates manufactured appropriate for every helpful purpose. To extract the dna, each component of the extraction mixture plays a part. During the dna extraction, the soap pulls apart the fats lipids and proteins that make up the membranes surrounding the cell and nucleus.
Students learn how genetic testing including dna extraction is useful in breeding new varieties of strawberries. Dna can be extracted from cells by a simple technique with household chemicals, enabling students to continue reading strawberry dna. Proficiently build strawberry dna extraction lab worksheet answers focus. Use a funnel and coffee filters to filter the strawberry juice into a beaker. Related posts of strawberry dna extraction lab worksheet answers light me up math worksheet answers before speaking about light me up math worksheet answers, be sure to are aware that knowledge will be our own answer to an improved down the road, plus studying does not only halt as soon as the education bell rings. Strawberries are an easy subject to use since they are readily obtained and processed and because they have multiple copies of their dna in each cell. How to extract strawberry dna with pictures wikihow. The dna precipitates out of the solution when the alcohol is added. The soap acts to dissolve the phospholipid bilayer of the cellular membrane and of the cells organelles. The independent variable in this experiment is the strawberry mixture while the dependent variable is the amount of. The long, thick fibers of dna store the information for the functioning of the chemistry of life.
Through this experiment we conclude that each and every. Apr 08, 2011 strawberry dna extraction lab formal write up essay dna extraction lab 11714 purpose. Dna extraction c 21 chemistry in the k8 classroom grades 48 2007, omsi orpapaya or pineapple juice. This simple method allows you to extract dna from a strawberry and view it. In this activity, students will learn the simple method of dna extraction, be able to explain the rationale of each step, and use simple household chemicals to extract dna from a strawberry. Students will learn how to extract this macromolecule from the fruit and will get to see it first. Dec 02, 2014 welcome to the fifteenth episode of season 2 of the sci guys. These special circumstances make strawberry dna both easy to extract and to see. Use 1 cup of fresh, frozen diluted as directed, or canned juice. Everyone is required to complete and submit the lab sheet upon. This lab was conducted in order to show and analyze the way dna is extracted. Dna extracting solution mix about 1 cup of dish detergent and 14 cup salt into 4. Once these membranes are broken apart, the dna is released from the cell.
Download strawberry dna extraction lab utah state university book pdf free download link or read online here in pdf. Strawberry dna extraction all living things have dna. We will show you how to mix a solution that will take apart the. Strawberry dna extraction background in this activity, you will extract a sample of dna from strawberries. Yet, its nearly impossible to grasp its importance if we cant see it. Nov, 2012 with the strawberry dna experiment, youll extract, isolate, and observe the dna of a strawberry in a matter of minutes. Though it is much too small to see on its own, it is visible to the human eye en masse. The dna found in strawberry cells can be extracted using common, everyday materials. It can easily be seen with the naked eye when collected from thousands of cells.
Make sure juice contains raw, uncooked fruit juice. Human cells are generally diploid, meaning they have two sets of chromosomes. Notes and hints keep the isopropyl alcohol very colduse the freezer or ice bucket. This incredible molecule can be easily seen with the naked eye when.
This lesson plan is for the extraction of dna from strawberries. Humans have two copies of dna in each cell except gametes and red. Learn science strawberry dna extraction with free interactive flashcards. Give to students as close to the start of the activity as possible. Strawberry dna extraction everything you need to teach a hands on laboratory investigation on the structure and function of dna. Dna lab objectives i will extract dna from the fruits that we eat such as strawberry by following dna extraction protocol. All books are in clear copy here, and all files are secure so dont worry about it. Answer any questions from the list made at the beginning of class. This is a digital download, of the classic strawberry dna extraction lab.
Place a strawberry in a zipclosure bag and remove most of the air before you seal the bag. Before speaking about light me up math worksheet answers, be sure to are aware that knowledge will be our own answer to an improved down the road. See lab handout strawberry dna extraction questions. The strawberry dna extraction activity, lab protocol and student worksheets were modified and used with permission from julie townsend, parkview middle school, ankeny, ia. Strawberries are a great choice for a dna extraction lab because they are octoploid, meaning that they have eight copies of each type of dna chromosome. This is specifically helpful for students being homeschooled if an educator wants to do the same lab but differentiated worksheets for each students age. This dna extraction lab guides students through learning about the cell membrane and dna all while observing dna they extracted from a strawberry. The purpose of this lab was to extract dna from a strawberry. Have students complete the student response worksheet. Strawberries are an exceptional fruit to use for this lesson because each individual student is able to complete the process by themselves and strawberries yield more dna than any other fruit i. It sounds impossible, but thanks to special characteristics of strawberries. Dec 11, 2015 strawberry dna extraction lab background. To keep everyone engaged, i make sure each student got their very own strawberry to smush. Dna is the representation of each and every living organism.
Strawberry dna can you extract dna from a strawberry. The salt acts to break up the protein chains around the nucleic acids, thus allowing for the dna extraction. Related posts of strawberry dna extraction lab worksheet answers light me up math worksheet answers. This incredible molecule can be easily seen with the naked eye when collected from thousands of cells. It can be concluded that from my experiment and the research that dna from different foods does vary and in appearance as well. The dna was extracted due to the dishwashing liquiddetergent busts open the cells of the fruit, releasing the dna and the salt helps as it ensures that the proteins in the cells are not separated from the rest of the solution with the dna. For the most part, students preferred the hands on lab because they were able to touch the materials. Each cell in a strawberry contains 8 copies of its genetic information. Read online strawberry dna extraction lab utah state university book pdf free download link book now. Dna is present in every cell of plants and animals. Strawberry dna extraction activity institute for molecular. Dna extraction c 20 chemistry in the k8 classroom grades 48 2007, omsi item amount needed strawberries 1 per group sealing plastic bags e.
Each cell in a plant or animal has a nucleus with multiple chromosomes. Steps i will explain the purpose of each step in the extraction procedure as it relates to the physical and biochemical characteristics of cells. Dna extraction lab for strawberries step by step guide. Apr 10, 2020 download strawberry dna extraction lab utah state university book pdf free download link or read online here in pdf. I predict that dna can be extracted and isolated from strawberry cells by using a dna extraction buffer and alcohol. Choose from 102 different sets of science strawberry dna extraction flashcards on quizlet. Doc dna extraction of strawberry and banana jefferson. Back to strawberry dna extraction lab worksheet answers. As a result, strawberries contain large amounts of dna. Strawberry dna extraction lesson plan primary learning. Eighth grade lesson strawberry dna extraction betterlesson. In this episode we are extracting the dna from a strawberry.
A teaspoons of the dna extraction liquid into the bag with the strawberries. With the strawberry dna experiment, youll extract, isolate, and observe the dna of a strawberry in a matter of minutes. The dna found in strawberry cells can be extracted using common, everyday. In the first part of the lab, we prepared a dna extraction buffer by mixing liquid dish detergent, salt, and distilled water together. This experiment show that dna can be extracted in many different ways from the simplest to the most complex way of extracting it. In this lab, students will have a blast extracting dna from a strawberry using common house hold materials to better understand its structure and function. The actual laboratory activity allows students to determine if dna is in the food we eat by attempting to extract dna from foods such as strawberries, onions, and bananas. Dna can be extracted from cells by a simple technique with household chemicals, enabling students to. In the first part of the lab, we prepared a dna extraction buffer by mixing liquid dish detergent, salt, and distilled. Strawberries are an exceptional fruit to use for this lesson because each individual student is able to complete the process by themselves and strawberries yield. Strawberry dna is easy to extract because strawberries are easy to mash, and ripe strawberries produce enzymes that contribute to the breakdown of cell walls. In this lab, you will extract strands of dna from the nuclei of strawberry.
Strawberry breeding and genetics utah state university. Tilt theu and pick up the dna using a plastic coffee stirrer or wooden stick. Strawberry dna extraction noble research institute. Strawberry dna extraction lesson plan this lesson plan is for the extraction of dna from strawberries.
Then alcohol is added to cause the dna to precipitate out. Dna, or deoxyribonucleic acid, is the basic programming in the cells of all living organisms. Ask about strawberry allergy 1 quart size ziplock bag 1 large or 2 small strawberries 2 teaspoons of extraction liquid 2 teaspoons of. This lab is fun and exciting and one students love. The salt enables the dna strands to come together, or aggregate. Strawberries are chosen for this particular experiment because strawberry cell has eight copies of the genome, and it would be easier to extract the dna since it is. The students also said they felt it was helpful to have completed the online extraction prior to completing the strawberry dna extraction. To understand how dna extraction is done, you will extract dna from strawberries today. I also give every student a copy of this free dna extraction lab student sheet also. Ahead of dealing with strawberry dna extraction lab worksheet answers, remember to are aware that instruction will be our key to an even better another day, along with understanding wont only end once the classes bell rings. Dna extraction the concentrations of the extracted dna was measured using the nanophotometer,uvvis spectrophotometer implen, munich, germany and the average concentration for 12 samples was 0.
Each component of the extraction mixture plays a part. Strawberry breeding and genetics student handout, 1 per student. If the lab is conducted properly then we should be able to view a visible amount of dna from the strawberry and detergent mixture. Apr 21, 2017 strawberry dna extraction adapted from a lab by c. Salt is added to release the dna strands by breaking up protein chains that hold nucleic acids together. My lab includes a student friendly lab sheet, teacher directions and a rubric for summative grading. Frozen strawberries should be thawed at room temperature. Dna isolation from strawberries student directions materials per student group strawberries about the volume of a golf ball. File type pdf strawberry extraction lab answers strawberry extraction lab answers strawberry dna extraction strawberry dna extraction is a fun experiment that you can perform using everyday materials.